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نویسندگان: Pegah Zanjanchi, Seyed Mohsen Asghari, Mostafa Shourian, Hassan Mohabatkar

زمان بندی: بدون زمان بندی

یونیت نمایش:

برچسب: Nanobiotechnology

کد: GP-42790

خلاصه مقاله: Abstract: Preventing the binding of vascular growth factors....

Abstract: Preventing the binding of vascular growth factors (VEGFs) to their receptors (VEGFRs) is considered an effective therapeutic approach based on their important role in the stimulation of angiogenesis and then tumor growth and metastasis. The purpose of this study is to increase the longevity of the designed VEGF antagonistic peptide (VGB3) in the circulation of the bloodstream by the use of gold nanoparticles (GNP). The modified GNPs were synthesized with the appropriate size (15-30 nm) and then EDC/NHS applied to activate carboxylic acid groups on the GNP surface to react with terminal amine groups of peptide. After that, the properties of conjugated nanoparticles, including the size, their binding confirmation, and biological activity in the in vivo and in vitro models (proliferation, migration, and apoptosis) were examined. In vivo conditions, the inhibition of breast tumor growth in Balb/c mouse models was studied by a suitable cancer model. The results showed a strong decrease in proliferation, and migration and a significant increased apoptosis of HUVECs and 4T1 cells being affected by free VGB3 and GNP-VGB3. Subsequently, the tumor growth inhibited in Balb/c mouse treated with GNP-VGB3 (0.5 mg/kg/week) more than other treatments in comparison with control. The final aim was to use the new peptide as a drug for the treatment of breast cancer by conjugation to GNP which can be entered into paramedics by reducing the drug dose rate and increasing the durability of peptide in the circulation of the bloodstream. Keywords: Angiogenesis, gold nanoparticles, VEGF, apoptosis, proliferation.


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