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نویسندگان: Alaleh Mohammadi, Marzie Hejazy , Masoumeh Firouzamandi

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برچسب: Nanomedicine

کد: GP-12370

خلاصه مقاله: Nowadays, the application of nanotechnology in various stages of food production, processing and packaging has gained....

Nowadays, the application of nanotechnology in various stages of food production, processing and packaging has gained special prominence.Nano silicon is a nano particle used as an antibacterial food additive in animal feed, poultry and in food packaging. Given the potential of this nano material to enter the human food chain, it is important to evaluate its risk for its side effects and toxicity. The purpose of this study was to investigate the effect of Nano Silicon on one of the apoptotic marker. For this purpose, 20 female rats were separated into 4 groups of 5 each and received 300, 600 and 900 mg/kg/day of Nano Silicon at 20-30 nm size orally. On the 20th day the rats were euthanized and their liver and kidneys were removed and transferred to a temperature at -80°C.The cDNA synthesized using specific primers was used to amplify the GLUT-2 gene and take into account a housekeeping gene in real-time PCR. Expression of CASPASE-3 protein in liver and kidney was evaluated by Real Time-PCR. Relative expression of CASPASE-3 gene in liver and kidney between different groups showed that CASPASE-3 gene expression in the liver showed the highest expression in the 300 and 900 mg Nano-silica receiving groups and the same expression in kidney cells. Keywords: Apoptosis, CASPASE-3, Real Time-PCR.


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