بدون زمان بندی
نویسندگان: Nazanin Biari, Dr. Ali Badiee, Dr. S.Hoda Alavizadeh
زمان بندی: بدون زمان بندی
خلاصه مقاله: Abstract: Whole Leishmania lysate antigens (WLL) have appeared effective to tackle leishmaniasis in murine models. The....
Abstract: Whole Leishmania lysate antigens (WLL) have appeared effective to tackle leishmaniasis in murine models. The activity of phospholipase-A (PLA) in WLL, prevents preparing stable liposomal WLL. One strategy to overcome this shortcoming is applying lipids such as sphingomyelin (SM) which is resistant against PLA. This study aim is formulating stable SM liposomes containing WLL and comparing their efficacy with another first generation vaccine, i.e. SLA liposomes in BALB/c mice. Methods: BALB/c mice were immunized subcutaneously, three times with 2-week intervals, with Empty-liposome (E-lipo), Particulate WLL, Liposome-WLL, Liposome-SLA and control Buffer, three times every 2-week. Protection was assessed through measuring the swollen footpads and the load of parasites in the spleen as well as other factors including IgG subclasses, IL-4 and IFN-γ levels and intracellular cytokine assay in cultured splenocytes. Results: Mice received Liposome-WLL did not reduce footpad swelling. Parasites presence in spleen and levels of IL-4 were also higher compared to other immunized groups. IgG isotypes, were not considerably different in mice received Liposome-WLL or other formulations.