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نویسندگان: Saba Talebian, Masoud Nejabat, Bahar Shahnavaz, Fatemeh B. Rassouli

زمان بندی: بدون زمان بندی

یونیت نمایش:

برچسب: Nanomedicine

کد: GP-06531

خلاصه مقاله: Introduction: Colon cancer is the third most common cancer and the second cause of cancer-associated deaths worldwide.....

Introduction: Colon cancer is the third most common cancer and the second cause of cancer-associated deaths worldwide. Among the various metal oxide nanoparticles, copper oxide nanoparticles (CuONPs) were noticed for their toxic activity and anticancer effects of these nanoparticles have been reported in a wide range of human cancer cell lines. In the present study, the potential toxicity of biogenic CuONPs was evaluated in human colon cancer cells, and compared with copper sulfate (CuSO4) salt. Methods: CuONPs were synthesized by Stenotrophomonas sp. BS95 and extracted after centrifugation. LoVo cells, a human colon adenocarcinoma cell line, were seeded at a density of 14000 cell/well in 96-well plates and treated with 50, 100 and 200 µg/ml of CuONPs for 24 h. To have a good evaluation of CuONPs bioactivity, copper sulfate (CuSO4) salt was used as control. The alamarBlue assay was used to determine viability of cells in each treatment. Results and conclusion: Viability assessment of LoVo cells indicated that 39% and 78% of cells were alive upon treatment with 50 µg/ml CuONPs and CuSO4, respectively. In addition, viability of cells was calculated as 27% and 57% upon treatment with 100 µg/ml CuONPs and CuSO4, respectively. Nevertheless, 200 µg/ml CuONPs and CuSO4 induced same effects, which might be due to their sedimentation in the highest concentration. According to our findings, biosynthesis of CuONPs greatly improved toxic activity of CuSO4 in colon cancer cells.Therefore, biogenic CuONPs could be considered as potent options for anticancer studies.


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