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نویسندگان: Samira Mozaffari, Esmat Alemzadeh, Saman Seyedabadi

زمان بندی: بدون زمان بندی

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برچسب: Nanomedicine

کد: GP-05174

خلاصه مقاله: In this study, we fabricated a doxorubicin-Berberine sustained release system using polycaprolactone-based electrospun....

In this study, we fabricated a doxorubicin-Berberine sustained release system using polycaprolactone-based electrospun fibers and evaluated their concomitant cytotoxic effects on MCF-7 breast cancer cells. We also investigated the effect of nanofibers of DOX and BBR alone and in combination on apoptosis induction of MCF7 cell line by Real-time PCR.in BCL2 and Caspase3 genes. The composite nanofibers of Poly-caprolactone containing anticancer drug DOX and BER were fabricated by electrospinning method that was divided into four groups: Group I: nanofiber maintained in drug-free environment as untreated control, Group II: nanofiber treated with different concentrations of DOX, Group III: nanofiber treated with various concentrations of BER. Group IV: nanofiber treated with different concentrations DOX and BER, We further extracted total RNA from MCF-7 cells, and RT-PCR assayed the expression of BCL2 and Caspase3 genes. The drug release profile was investigated by UV-Vis spectrophotometer at different pH (5.4 and 7.4) and 37.5 ̊C. The surface morphology and physicochemical properties of nanofibers were also characterized using FTIR and SEM. In vitro drug release experiments showed that the doxorubicin release at pH=5.4 was promisingly more and faster than drug release at pH= 7.4. MTT assays also indicated that the BER-DOX-loaded nanofibers were more toxic than DOX noanofiber and BER nanofibers alone. The combination of DOX and BER indicated synergistic anticancer effects on MCF-7 cell. The BER -DOX-loaded nanofibers showed a controlled and sustained release profile in vitro up to 72 h.


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